Rapid and simple detection of the invA gene in Salmonella spp. by isothermal target and probe amplification (iTPA)
نویسندگان
چکیده
منابع مشابه
Selective Amplification of prt, tyv and invA Genes by Multiplex PCR for Rapid Detection of Salmonella typhi
A multiplex PCR-based assay was developed for detection of Salmonella typhi and identification of other salmonella serotypes. Three primer-sets were selected from different genomic sequences, malo2-F/malo2-Ra primers from invasion gene, Parat-s/Parat-as as well as tyv-s/tyv-as primers from O-antigen gene cluster of the genus Salmonella. This method differentiated Salmonella spp., based on size ...
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Species identification of animal tissues in meat products is an important issue to protect the consumer from illegal and/or undesirable adulteration, for economic, religious and health reasons. For example, pork DNA was found in halal chicken sausages served in a primary school in Westminster, central London, the local authority had stated on 14 March 2015. PCR is one of the most widely used te...
متن کاملDetection of Salmonella spp. from some wild captive herbivores in Iran and determination of serogroup, antibiotic susceptibility and presence of invA gene in the isolated strains
Salmonella spp. are zoonotic enteric bacteria able to infect humans, livestock and wildlife. The aim of this study was to investigate the prevalence of Salmonella (spp.) and to determine antibiotic susceptibility, serogrouping and presence of invA gene (Salmonella Invasion Gene A) in the detected strains in wild captive herbivores in Iran. The fecal samples of 103 animals from 8 different speci...
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conclusions the analytical sensitivity of lamp and pcr methods, using the designed primers, was 8 times more than any other reported methods. the designed methods are specific and sensitive for detection of h. pylori in different clinical and environmental samples. results the detection limits of the lamp and pcr assay were similar and were 10 fg of pure dna of h. pylori, which is equal to 6 co...
متن کاملselective amplification of prt, tyv and inva genes by multiplex pcr for rapid detection of salmonella typhi
a multiplex pcr-based assay was developed for detection of salmonella typhi and identification of other salmonella serotypes. three primer-sets were selected from different genomic sequences, malo2-f/malo2-ra primers from invasion gene, parat-s/parat-as as well as tyv-s/tyv-as primers from o-antigen gene cluster of the genus salmonella. this method differentiated salmonella spp., based on size ...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2011
ISSN: 0266-8254
DOI: 10.1111/j.1472-765x.2011.03018.x